Publications

Publications

2026-present

S Dupuis1, E Forster1, C Ialy-Radio, VHB Serrão, P Yip, M Foritano, S Barbaux, JE Lee* and A Ziyyat*. ERp57 is crucial for mammalian fertilization but functions independently of IZUMO1. Cellular and Molecular Biology Letters. June 27 1co-first authors; *co-senior author

MS Girault, S Dupuis, R Pierre, C Gourier, JE Lee, S Barbaux and A Ziyyat. ADAM20 participates modestly to human fertilization and its absence leads to in vitro hypofertility in mouse. Cell Biochemistry & Function 44(7): e70265.

P Cots-Rodríguez, X Wang, M Sanchez-Tudela, KK Siu, P Yip, E Gómez, JE Lee, JG Hamze and M Jiménez-Movilla. (2026). JUNO-coated beads as a functional assay to capture and characterize fertilization-competent human sperm. Human Reproduction Open. 2026(1):hoag010. 

2021-2025

E Forster1, X Wang1, A Thayaparan1, and JE Lee. (2025). SnapShot: Human sperm-egg interface proteins. Cell. 188(6) 1746. (SRA) 1co-first authors

S Dupuis, M Le Beulze, TDR Vance, R Pierre, JE Lee*, A Ziyyat* and S Barbaux*. (2025). Spermatozoa lacking TEX51 display hypofertility and defects in morphology. Communications Biology. 8(1):1563. (co-SRA) *co-senior author

CC Taber, W He, GMC Gasmi-Seabrook, M Hubert, FG Ferens, M Ikura, JE Lee and M Ohh. (2025). Erythrocytosis-inducing PHD2 mutations implicate biological role for N-terminal prolyl-hydroxylation in HIF1α oxygen-dependent degradation domain. Elife. 20:14:RP107121.

JK Bruce, LY Li, Y Tang, E Forster, NJ Winsor, PY Bi, C Krustev, S Keely JE Lee, JR Rohde, HY Gaisano, DJ Philpott, and SE Girardin. (2025). Gasdermin-D pores induce an inactivating caspase-4 cleavage that limits IL-18 production in the intestinal epithelium. Communications Biology. 8(1):737.

W He, GMC Gasmi-Seabrook, M Ikura, JE Lee*, and M Ohh*. (2024). Time-resolved NMR detection of prolyl-hydroxylation in intrinsically disordered region of HIF1a. PNAS. 121(37):e2408104121 *co-senior author

VE Deneke, A Blaha, Y Lu, JM Draper, CS Phan, K Panser, A Schleiffer, L Jacob, T Humer, K Stejskal, G Krssakova, D Handler, M Kamoshita, TDR Vance, X Wang, E Roitinger, JE Lee, M Ikawa, and A Pauli. (2024). A conserved fertilization complex bridges sperm and egg in vertebrates. Cell. 187(25) 7066-2078

S Ouzikov, KM Edwards, T Anadampillai, S Watanabe, D Lozano Casabuenas, K Siu, A Dou, D Jeong, JE Lee, and SA Yuzwa. (2024). LRIG1 controls proliferation of adult neural stem cells by facilitating Tgfb and BMP signalling pathways. Communications Biology. 7(1):845

FG Ferens, CC Taber, S Stuart, M Hubert, D Tarade, JE Lee, and M Ohh. (2024). Deficiency in PHD2-mediated hydroxylation of HIF2alpha underlies Pacak-Zhuang syndrome. Communications Biology. 7(1):240

TDR Vance, P Yip, E Jiménez, S Li, D Gawol, J Byrnes, I Usón, A Ziyyat, and JE Lee. (2022). SPACA6 ectodomain structure reveals a conserved superfamily of gamete-fusion associated proteins. Communications Biology. 5(1):984.

JD Cook, A Khondker, and JE Lee. (2022). Conformational plasticity of the HIV-1 gp41 immunodominant region is recognized by multiple non-neutralizing antibodies. Communications Biology. 5(1):291.

TT Dean, VHB Serrao, and JE Lee. (2022). Structure of the core postfusion porcine endogenous retrovirus fusion protein. mBio. 13(1):e0292021.

W He, S Batty-Stuart, JE Lee, M Ohh. (2021) HIF-1α Hydroxyprolines Modulate Oxygen-Dependent Protein Stability Via Single VHL Interface With Comparable Effect on Ubiquitination Rate. J Mol Biol. 433(22):167244.

T Gebregiworgis, Y Kano, J St-Germain, N Radulovich, ML Udaskin, A Mentes, R Huang, BPK Poon, W He, I Valencia-Sama, CM Robinson, M Huestis, J Miao, JJ Yeh, ZY Zhang, MS Irwin, JE Lee, MS Tsao, B Raught, CB Marshall, M Ohh, M Ikura. (2021) The Q61H mutation decouples KRAS from upstream regulation and renders cancer cells resistant to SHP2 inhibitors. Nat Commun. 12(1):6274

KK Siu, VHB Serrão, A Ziyyat, JE Lee. (2021). The cell biology of fertilization: Gamete attachment and fusion. J. Cell Bio. 220(10):e202102146

VHB Serrao, JD Cook and JE Lee. (2021). Snapshot of an influenza virus glycoprotein fusion intermediate. Cell Reports. 35(7):109152

P Mastrangelo, AA Chin, S Tan, AH Jeon, CA Ackerley, KK Siu, JE Lee, Hegele RG. (2021). Identification of RSV Fusion Protein Interaction Domains on the Virus Receptor, Nucleolin. Viruses. 13(2):261

2016-2020

VHB Serrao and JE Lee. (2020). FRETing over SARS-CoV-2: conformational dynamics of the spike glycoprotein. Cell Host Microbe. 28(6):778-79.

J Mercer, JE Lee, EO Saphire and SA Freeman. (2020). Snapshot: Enveloped virus entry. Cell. 182(3):786.

TDR Vance and JE Lee. (2020). Virus and eukaryote fusogen superfamily. Current Biology. 30(13):R750-54.

FC Azimi and JE Lee. (2019). Structural perspectives on HIV-1 Vif and APOBEC3 restriction factor interactions. Protein Sci. 29(2):391-406.

D Tarade, JE Lee and M Ohh. (2019). Evolution of metazoan oxygen-sensing involved a conserved divergence of VHL affinity for HIF1α and HIF2α. Nat Commun. 10(1):3293.

Y Kano, T Gebregiworgis, CB Marshall, N Radulovich, BPK Poon, J St-Germain, JD Cook, I Valencia-Sama, BMM Grant, SG Herrera, J Miao, B Raught, MS Irwin, JE Lee, JJ Yeh, ZY Zhang, MS Tsao, M Ikura, M Ohh. (2019). Tyrosyl phosphorylation of KRAS stalls GTPase cycle via alteration of switch I and II conformation. Nat Commun. 10(1):224.

C Jean, F Haghighirad, Y Zhu, M Chalbi, A Ziyyat, E Rubinstein, C Gourier, P Yip, JP Wolf, JE Lee, C Boucheix, V Barraud-Lange. (2019). JUNO, the receptor of sperm IZUMO1, is expressed by the human oocyte and is essential for human fertilisation. Human Reprod. 34(1):118-126.

D Tarade, CM Robinson, JE Lee, M Ohh. (2018) HIF-2α-pVHL complex reveals broad genotype-phenotype correlations in HIF-2α-driven disease. Nat Commun. 9(1):3359.

JD Cook, A Sultana, and JE Lee. (2017). Structure of the infectious salmon anemia virus receptor complex illustrates a unique binding strategy for attachment. Proc Natl Acad Sci U S A. 114(14): E2929-2936.

H Aydin, A Sultana, S Li, A Thavalingam, and JE Lee. (2016). Molecular architecture of the human sperm izumo1 and egg Juno fertilization complex. Nature. 534(7608):562-5.

KJ Abraham, JN Chan, JS Salvi, B Ho, A Hall, E Vidya, R Guo, SA Killackey, N Liu, JE Lee, GW Brown, and K Mekhail. (2016). Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids. Nucleic Acid Res. 44(18):8870-8884.

Y Kano, JD Cook, JE Lee, and M Ohh. (2016). New structural and functional insight into the regulation of Ras. Semin. Cell. Dev. Biol. 58:70-78.

P Heir, T Srikumar, G Bikopoulos, S Bunda, BP Poon, JE Lee, B Raught, and M Ohh. (2016). Oxygen dependent regulation of erthyropoietin receptor turnover and signaling. J Biol Chem. 291(14):7357-72.

2011-2015

JD Cook, H Soto-Montoya, MK Korpela, and JE Lee. (2015). Electrostatic architecture of the ISAV core fusion protein illustrates carboxyl-carboxylate pH sensor.  J Biol Chem. 290(30):18495-504.

T Hashiguchi, ML Fusco, ZA Bornholdt, JE Lee, AI Flyak, R Matsuoka, D Kohda, Y Yanagi, M Hammel, JE Crowe Jr, and EO Saphire. (2015). Structural basis of Marburg virus neutralization by a cross-reactive human antibody. Cell. 160(5):904-12.

A Sultana and JE Lee. (2015). Measuring protein-protein and protein-nucleic acid interactions by biolayer interferometry. Curr Protoc Protein Sci. 79:19.25.1-26.

S Bunda, P Heir, T Srikumar, JD Cook, K Burrell, Y Kano, JE Lee, G Zadeh, B Raught, and M Ohh. (2014). Src promotes GTPase activity of Ras via tyrosine 32 phosphorylation. Proc Natl Acad Sci U S A. 111(36):E3785-94.

H Aydin*, M Taylor* and JE Lee. (2014). Structure-guided analysis of the human APOBEC3-HIV restrictome. Structure. 22(5):668-84.

H Aydin, D Al-Khooly and JE Lee. (2014). Influence of hydrophobic and electrostatic residues on SARS-coronavirus S2 protein stability: insights into mechanisms of general viral fusion and inhibitor design. Protein Science. 23(5):603-17.

KK Siu, A Sultana, FC Azimi and JE Lee. (2013). Structural determinants of HIV-1 Vif susceptibility and DNA binding in APOBEC3F. Nature Communications. 4 (4), 2593.

H Aydin, JD Cook and JE Lee. (2013). Crystal structures of beta- and gamma-retroviral fusion proteins reveal a role for electrostatic stapling in viral entry. Journal of Virology. 88 (1)143-53.

H Aydin, BM Smrke and JE Lee. (2013). Structure of a retroviral fusion protein from a virus that undergoes a two-step viral entry mechanism. FASEB Journal. 27 5072-82.

JD Cook and JE Lee. (2013). The secret life of viral entry glycoproteins: moonlighting in immune evasion. PLoS Pathogens.  9 (5) e1003258. 1-5.

P Heir, RI Sufan, SN Greer, BP Poon, JE Lee, and M Ohh. (2013). DCNL1 functions as a substrate sensor and acivator of cullin2-RING ligase. Mol Cell Biol 33 (8),1621-31.

H Aydin*, FC Azimi*, JD Cook*, and JE Lee. (2012).  A convenient and general expression platform for the production of secreted proteins from human cells. Journal of Visualized Experiments. 65. pii: 4041.

2006-2010 (publications prior to UToronto)

JE Lee, ML Fusco, and EO Saphire.  (2009). An efficient platform for screening expression and crystallization of glycoproteins in human cells.  Nature Protocols. 4 (4), 592-604

JE Lee, ML Fusco, DM Abelson, AJ Hessell, DR Burton and EO Saphire. (2009).  Techniques and tactics used in determining the structure of the trimeric Ebolavirus glycoprotein.  Acta Crystallographica D65, 1162-1180.

JE Lee and EO Saphire. (2009). Neutralizing Ebolavirus: structural insights into the envelope glycoprotein and antibodies targeted against it.  Current Opinions in Structural Biology.  19, 1-10.

JE Lee and EO Saphire. (2009).  Ebolavirus glycoprotein structure and mechanism of entry.  Future Virology. 4 (6), 621-635.

JE Lee, ML Fusco, AJ Hessell, WB Oswald, DR Burton, and EO Saphire.  (2008).  Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor.  Nature. 454, 177-182.  (featured cover article)